Take the liquid over the column but keep the packing wet as the standard, just follow the parameters of the instruction manual. If the whole tube consists of a large amount of liquid not centrifuged down, you can repeat the centrifugation a few times, or consider that it may be caused by too high a concentration of protein, you need to dilute the sample and take 1-10 μg of protein on the sample.

No. Self-adding iRT is available.Additionally Dissolve B can be replaced with solvents commonly used in the laboratory to re-solubilize peptides.

For timsTOF instruments (Bruker), dissolve the peptide to 200 ng/μL and inject 1 μL. If the initial sample size is less than 500 ng, it is recommended that 5 μL of Dissolve B be used to dissolve the sample and 4 μL be injected; For Orbitrap instruments (Thermo), dissolve peptide to 1 μg/μL of sample, e.g., for an initial sample of 10 μg, dissolve peptide in 10 μL of Dissolve B and inject 1-3 μL. If the initial sample size is less than 2 μg, it is recommended to dissolve peptide in 5 μL of Dissolve B and inject 4 μL of peptide.

Yes, reuse is recommended. At the end of each experiment, the Adaptor should be submerged in anhydrous ethanol and ultrasonically cleaned (in a special glass beaker), dried naturally and then stored in a special glass beaker and sealed with tinfoil until the next use.

Use within 3h after reconstitution, it is not recommended to keep it for reuse.