Firstly, a large range of ROI can be identified through panoramic imaging, that is, the whole film is partitioned, and a certain large ROI is selected as the microdissection range of the collected sample. Then, through the intelligent analysis algorithm, based on the multi-dimensional characteristics of the microdissection target (maybe a certain cell type, blood vessels or interstitium) such as morphology, size, fluorescence intensity, etc., the target small ROI in the selected large ROI is identified.

When performing microdissection, from top to bottom it should be a sandwich structure of "membrane-sample-cover glass". The sample is between the membrane and the coverslip to ensure that the sample is clean and the entire process is contamination-free.

The kit can be sent at room temperature. Long-term storage suggestions: reagents and enzymes can be stored at -20 ℃, consumables (independent small packages) are placed at room temperature separately, to avoid the consumables to be refrigerated to room temperature when moisture absorption and affect the results of the experiment.

Plant samples are recommended to use acetone for protein precipitation after protein extraction and washing before protein quantification.

Changes in pH can cause precipitation of certain proteins and prolonged storage of acidified samples is not recommended.

No, a total volume of 20-50 μL is sufficient. You can load up to 2 times for a total of about 60 μl: 60 μl can be added to 6 μl of Acidize, aliquoted in two sequential samples, i.e. 33 μl at the loading step, centrifuged, and then repeated with 33 μl, centrifuged.

When using the Reduce reagent, the recommended optimal reduction time is 15 min and the maximum reduction time should not exceed 60 min.

To set the minimum centrifuge time, manually click Stop 5 sec after centrifugation has started.