The innovative introduction of multi-color immunofluorescence, and at the same time compatibility with multiple staining methods such as HE staining, and by integrating the automated full-field AI intelligent recognition imaging system, positioning system and microdissection system into one, were all implemented to execute automated precise positioning, navigation and cutting. While performing high-depth proteome detection, precise spatial position information with single-cell resolution is obtained, spatial visualization of target cell types is achieved and target protein expression is spatially visualized.

Based on spatially visualized proteome-specific film, the two systems of staining imaging and microdissection can be connected to achieve the whole process of spatially visualized proteome detection on the same tissue slice. Therefore, you should contact us to obtain the special films for spatially visualized proteomics for your formal samples. Samples that have already been placed on the general pathological glass slide cannot go through the whole process of spatially visualized proteomic detection.

The spatially visualized proteomics project focuses on clear target areas or specific cell types. To determine whether the slice area can be accurately located, whether the correct target area/cell type can be distinguished, and whether an executable microdissection plan can be further developed, it is necessary to determine the slice direction and slice depth, as well as the slice staining method and imaging method through the preparations. In summary, the preliminary experiment process for starting with a tissue block sample includes four steps: slicing, staining, imaging and analysis. The preparation is somewhat exploratory and may have a long cycle. Starting from the stained slice sample (and the slice is attached to a special membrane for spatial proteomics), only two steps are required: imaging and analysis. After the imaging quality control analysis is qualified, the formal experiment can be carried out immediately.

(1) Compare different regions of the same section, i.e., multiple tubes of samples can be cut and collected on the same section to compare protein expression profiles between different tissue microenvironment regions/different cell types. Or (2) compare the change of same region of tissue slices across spatiotemporal states or of different experimental preparations.

We recommend at least three repetitions, which can be in the form of either technical repetition or biological repetition. One sample is the sample in one test tube from microdissection. One tube of sample of microdissection is recommended to have a total area of 0.2 mm2 or greater, which is usually a few to a few dozen small targeted cutting regions/total area of the same slice.

It is recommended to use either paraffin or OCT embedding. For paraffin embedding, FFPE samples generally have better staining imaging quality, and the slices should be 4 μm and transported at 4 ℃. For OCT embedding, generally OCT frozen tissue samples have slices of 10 μm and need to be transported with dry ice.

Yes, and it is recommended that more than 5 consecutive slices of each sample to be provided for preparation, formal experiment, and back-up. The samples need to be on special films for spatially visualized proteomics, and should avoid any scratches, wrinkles, or impurities from the film which can affect imaging quality and cell location.