The kit can be sent at room temperature. Long-term storage suggestions: reagents and enzymes can be stored at -20 ℃, consumables (independent small packages) are placed at room temperature separately, to avoid the consumables to be refrigerated to room temperature when moisture absorption and affect the results of the experiment.

Plant samples are recommended to use acetone for protein precipitation after protein extraction and washing before protein quantification.

Changes in pH can cause precipitation of certain proteins and prolonged storage of acidified samples is not recommended.

No, a total volume of 20-50 μL is sufficient. You can load up to 2 times for a total of about 60 μl: 60 μl can be added to 6 μl of Acidize, aliquoted in two sequential samples, i.e. 33 μl at the loading step, centrifuged, and then repeated with 33 μl, centrifuged.

When using the Reduce reagent, the recommended optimal reduction time is 15 min and the maximum reduction time should not exceed 60 min.

To set the minimum centrifuge time, manually click Stop 5 sec after centrifugation has started.

Take the liquid over the column but keep the packing wet as the standard, just follow the parameters of the instruction manual. If the whole tube consists of a large amount of liquid not centrifuged down, you can repeat the centrifugation a few times, or consider that it may be caused by too high a concentration of protein, you need to dilute the sample and take 1-10 μg of protein on the sample.

No. Self-adding iRT is available.Additionally Dissolve B can be replaced with solvents commonly used in the laboratory to re-solubilize peptides.