Firstly, a large range of ROI can be identified through panoramic imaging, that is, the whole film is partitioned, and a certain large ROI is selected as the microdissection range of the collected sample. Then, through the intelligent analysis algorithm, based on the multi-dimensional characteristics of the microdissection target (maybe a certain cell type, blood vessels or interstitium) such as morphology, size, fluorescence intensity, etc., the target small ROI in the selected large ROI is identified.

When performing microdissection, from top to bottom it should be a sandwich structure of "membrane-sample-cover glass". The sample is between the membrane and the coverslip to ensure that the sample is clean and the entire process is contamination-free.